https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 Psychological Stress Management and Stress Reduction Strategies for Stroke Survivors: A Scoping Review https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50112 Wed 28 Feb 2024 16:20:34 AEDT ]]> The Regulation of Uterine Function During Parturition: An Update and Recent Advances https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39583 Wed 27 Jul 2022 10:45:46 AEST ]]> Methods and model systems used to study pregnant human uterine smooth muscle https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47519 Wed 24 Jan 2024 14:11:41 AEDT ]]> Modulation of progesterone receptor isoform expression in pregnant human myometrium https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30926 PR isoform expression ex situ and, further, to determine if conditions approaching the in vivo environment stabilise PR isoform expression in culture. Methods: Term nonlaboring human myometrial tissues were cultured under specific conditions: serum supplementation, steroids, stretch, cAMP, PMA, PGF2α, NF-κB inhibitors, or TSA. Following 48 h culture, PR-T, PR-A, and PR-B mRNA levels were determined using qRT-PCR. PR-A/PR-B ratios were calculated. Results: PR-T and PR-A expression and the PR-A/PR-B ratio significantly increased in culture. Steroids prevented the culture-induced increase in PR-T and PR-A expression. Stretch blocked the effects of steroids on PR-T and PR-A expression. PMA further increased the PR-A/PR-B ratio, while TSA blocked culture-induced increases of PR-A expression and the PR-A/PR-B ratio. Conclusion: Human myometrial tissue in culture undergoes changes in PR gene expression consistent with transition toward a laboring phenotype. TSA maintained the nonlaboring PR isoform expression pattern. This suggests that preserving histone and/or nonhistone protein acetylation is critical for maintaining the progesterone dependent quiescent phenotype of human myometrium in culture.]]> Wed 11 Apr 2018 14:38:10 AEST ]]> Regulation of expression of key genes in uterine myocytes https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30455 Wed 11 Apr 2018 09:37:03 AEST ]]> The expression of genes involved in myometrial contractility changes during ex situ culture of pregnant human uterine smooth muscle tissue https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34255 Ex situ analyses of human myometrial tissue has been used to investigate the regulation of uterine quiescence and transition to a contractile phenotype. Following concerns about the validity of cultured primary cells, we examined whether myometrial tissue undergoes culture-induced changes ex situ that may affect the validity of in vitro models. Objectives: To determine whether human myometrial tissue undergoes culture-induced changes ex situ in Estrogen receptor 1 (ESR1), Prostaglandin-endoperoxide synthase 2 (PTGS2) and Oxytocin receptor (OXTR) expression. Additionally, to determine whether culture conditions approaching the in vivo environment influence the expression of these key genes. Methods: Term non-laboring human myometrial tissues were cultured in the presence of specific treatments, including; serum supplementation, progesterone and estrogen, cAMP, PMA, stretch or NF-κB inhibitors. ESR1, PTGS2 and OXTR mRNA abundance after 48 h culture was determined using quantitative RT-PCR. Results: Myometrial tissue in culture exhibited culture-induced up-regulation of ESR1 and PTGS2 and down-regulation of OXTR mRNA expression. Progesterone prevented culture-induced increase in ESR1 expression. Estrogen further up-regulated PTGS2 expression. Stretch had no direct effect, but blocked the effects of progesterone and estrogen on ESR1 and PTGS2 expression. cAMP had no effect whereas PMA further up-regulated PTGS2 expression and prevented decline of OXTR expression. Conclusion: Human myometrial tissue in culture undergoes culture-induced gene expression changes consistent with transition toward a laboring phenotype. Changes in ESR1, PTGS2 and OXTR expression could not be controlled simultaneously. Until optimal culture conditions are determined, results of in vitro experiments with myometrial tissues should be interpreted with caution.]]> Wed 04 Sep 2019 10:05:05 AEST ]]> Epigenetic regulation of progesterone receptors and the onset of labour https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47796 Tue 31 Jan 2023 15:32:49 AEDT ]]> Understanding microglial involvement in stress-induced mood disturbance: a modulator of vulnerability? https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47791 Tue 31 Jan 2023 14:50:57 AEDT ]]> Exploring how low oxygen post conditioning improves stroke-induced cognitive impairment: a consideration of amyloid-beta loading and other mechanisms https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38036 Tue 27 Jul 2021 15:08:13 AEST ]]> Expression of KCNH2 (hERG1) and KCNE2 correlates with expression of key myometrial genes in term pregnant human myometrium https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32092 Mon 23 Sep 2019 12:35:23 AEST ]]> Histone deacetylase inhibitors: providing new insights and therapeutic avenues for unlocking human birth https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49910 Mon 19 Jun 2023 11:07:36 AEST ]]> Astrocyte remodeling in the beneficial effects of long-term voluntary exercise in Alzheimer's disease https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38402 Mon 06 Sep 2021 16:41:33 AEST ]]> Drug delivery to the human and mouse uterus using immunoliposomes targeted to the oxytocin receptor https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32098 Fri 03 Dec 2021 10:35:09 AEDT ]]>